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9. Contrast methods for Leica DM4000 B/DM4500 P/DM5000 B

9.1 Transmitted light

9.1.1 Bright field (TL)

• Switch to the transmitted light axis (TL) by

pushing the TL/IL function key.

• Select the BF (bright field) contrast method.

Do so by pressing the BF variable key.

Alternatively: press the variable button

CHANGE TL

(For details on key assignments, please see

the identification sheet.)

The display indicates BF.

• Insert a transmitted light specimen.

• Rotate an appropriate objective into place.

• Use the focus wheel to bring the image into

focus and set the brightness using the INT

function key.

9.1.2 Phase contrast (TL)

• Switch to the transmitted light axis (TL) by

pushing the TL/IL function key.

• Select the PH (phase contrast) contrast meth-

od.

Do so by pressing the variable button PH.

Alternatively: press the variable button

CHANGE TL

(For details on key assignments, please see

the identification sheet.)

The display indicates PH.

• Insert a transmitted light specimen.

• Rotate an appropriate objective into place.

Objectives that are suitable for phase con-

trast are engraved with PH.

• Bring the image into focus using the focus

wheel and set the brightness using the INT

function key.

Notes:

The microscope automatically selects the cor-

rect light ring in the condenser.

When selecting the phase contrast method, the

aperture diaphragm is opened fully and can not

be adjusted.

9. Contrast methods for Leica

DM4000 B/DM4500 P/DM5000 B

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