Sharp VN-EZ1 Manual de usuario

90013601037181QIAGEN GmbH, D-40724 HildenR1Version 1 February 2007Sample & Assay TechnologiesBioRobot®EZ1 DSP Workstation User Manual9001360103718

Safety Information 1-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 Voltage rating label The following label appears on the back of the BioRobot

Safety Information BioRobot EZ1 DSP Workstation User Manual 02/2007 1-5 1.4 Biological safety Specimens and reagents containing materials from huma

Safety Information 1-6 BioRobot EZ1 DSP Workstation User Manual 02/2007 1.5 Chemicals WARNING Hazardous chemicals [W6] Some chemicals used with

Safety Information BioRobot EZ1 DSP Workstation User Manual 02/2007 1-7 1.7 Mechanical hazards The worktable of the BioRobot EZ1 DSP moves during o

Safety Information 1-8 BioRobot EZ1 DSP Workstation User Manual 02/2007 1.9 Symbols on the BioRobot EZ1 DSP Symbol Location Description Heating

Safety Information BioRobot EZ1 DSP Workstation User Manual 02/2007 1-9 Symbol Location Description SN Type plate on the back of the instrument Seri

Safety Information 1-10 BioRobot EZ1 DSP Workstation User Manual 02/2007 Symbol on the Heating System Symbol near the Tip Rack

Introduction BioRobot EZ1 DSP Workstation User Manual 02/2007 2-1 2 Introduction Thank you for choosing the QIAGEN BioRobot EZ1 DSP workstation. We

Introduction 2-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 QIAGEN products. If you have any questions regarding the BioRobot EZ1 DSP or QIAGE

Introduction BioRobot EZ1 DSP Workstation User Manual 02/2007 2-3 2.3.1 Requirements for BioRobot EZ1 DSP users This table covers the general level

Trademarks: QIAGEN®, BioRobot®(QIAGEN Group); Gigasept®, Lysetol®, Mikrozid®(Schülke & Mayr GmbH); DECON-QUAT®(Veltek Associates, Inc.).© 2007 QIA

Introduction 2-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 This page intentionally left blank

General Description BioRobot EZ1 DSP Workstation User Manual 02/2007 3-1 3 General Description The BioRobot EZ1 DSP workstation performs fully auto

General Description 3-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 3.1 External features of the BioRobot EZ1 DSP Front View of the BioRobot E

General Description BioRobot EZ1 DSP Workstation User Manual 02/2007 3-3 Keypad The following keys of the control panel are available to the user.

General Description 3-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 could be lost, leading to a memory error. EZ1 Cards should not be exchanged

General Description BioRobot EZ1 DSP Workstation User Manual 02/2007 3-5 3.1.5 Connector panel The connector panel is located at the rear of the Bi

General Description 3-6 BioRobot EZ1 DSP Workstation User Manual 02/2007 3.2.1 Worktable The worktable contains various racks for holding the labwa

General Description BioRobot EZ1 DSP Workstation User Manual 02/2007 3-7 Tip Rack Both tip holders and filter-tips are made of polypropylene and a

General Description 3-8 BioRobot EZ1 DSP Workstation User Manual 02/2007 Cartridge rack This rack is located behind the tip rack and holds up to 6 r

General Description BioRobot EZ1 DSP Workstation User Manual 02/2007 3-9 Heating system The heating system is located under the far end of the cartr

Contents BioRobot EZ1 DSP Workstation User Manual 02/2007 Contents-1 Contents 1 Safety Information 1-1 1.1 Proper use 1-2 1.2 Electrical safety

General Description 3-10 BioRobot EZ1 DSP Workstation User Manual 02/2007 During operation of the BioRobot EZ1 DSP, the piercing unit first exposes

Installation Procedures BioRobot EZ1 DSP Workstation User Manual 02/2007 4-1 4 Installation Procedures The BioRobot EZ1 DSP is a plug-and-play inst

Installation Procedures 4-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 workstation. To preserve this protection feature, do not operate the wo

Installation Procedures BioRobot EZ1 DSP Workstation User Manual 02/2007 4-3 WARNING Electrical hazard [W12] Never install a fuse different from

Installation Procedures 4-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 2. Remove the outer top cardboard. 3. Take out the packages with t

Installation Procedures BioRobot EZ1 DSP Workstation User Manual 02/2007 4-5 7. Remove the inner top cardboard, and remove the BioRobot EZ1 DSP fro

Installation Procedures 4-6 BioRobot EZ1 DSP Workstation User Manual 02/2007 1. Cut and remove the cable tie. 2. Remove the bubble wrap and the

Installation Procedures BioRobot EZ1 DSP Workstation User Manual 02/2007 4-7 Note: It is recommended to save the original packaging material for lat

Installation Procedures 4-8 BioRobot EZ1 DSP Workstation User Manual 02/2007 This page intentionally left blank

General Operation BioRobot EZ1 DSP Workstation User Manual 02/2007 5-1 5 General Operation This section describes how to operate the BioRobot EZ1 D

Contents Contents-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 3.2 Internal features of the BioRobot EZ1 DSP 3-5 3.2.1 Worktable 3-6 3.2.

General Operation 5-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 5.2 Inserting and removing the EZ1 Card CAUTION Damage to the instrument

General Operation BioRobot EZ1 DSP Workstation User Manual 02/2007 5-3 Ensure that the card is completely inserted into the slot. It is extremely

General Operation 5-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 3. Press the button at the bottom of the EZ1 Card slot. The EZ1 Card is e

General Operation BioRobot EZ1 DSP Workstation User Manual 02/2007 5-5 4. After initialization, the main menu appears. You can now operate th

General Operation 5-6 BioRobot EZ1 DSP Workstation User Manual 02/2007 5.4.1 Opening the workstation door 1. Slide your fingers under the door and

General Operation BioRobot EZ1 DSP Workstation User Manual 02/2007 5-7 2. Start worktable setup by pressing the appropriate number key (e.g., “1” o

General Operation 5-8 BioRobot EZ1 DSP Workstation User Manual 02/2007 1. Press “STOP”. The protocol run pauses and the LCD displays the following

General Operation BioRobot EZ1 DSP Workstation User Manual 02/2007 5-9 5.6.1 Removing and replacing the tray The tray is located under the worktabl

General Operation 5-10 BioRobot EZ1 DSP Workstation User Manual 02/2007 4. Slide the reagent cartridges into the cartridge rack in the direction of

General Operation BioRobot EZ1 DSP Workstation User Manual 02/2007 5-11 5.6.3 Loading the elution tubes, filter-tips, and sample tubes 1. Remove t

Contents BioRobot EZ1 DSP Workstation User Manual 02/2007 Contents-3 6.3 Weekly maintenance procedure 6-5 6.4 Reagents for decontamination 6-6 7

General Operation 5-12 BioRobot EZ1 DSP Workstation User Manual 02/2007 6. Return the tip rack to the worktable. Ensure that the tips, tip hol

Preventive Maintenance BioRobot EZ1 DSP Workstation User Manual 02/2007 6-1 6 Preventive Maintenance The following preventive maintenance procedure

Preventive Maintenance 6-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 6.1 Regular maintenance procedure WARNING Risk of personal injury an

Preventive Maintenance BioRobot EZ1 DSP Workstation User Manual 02/2007 6-3 3. Press “3” to select “Clean pierce unit”. The piercing unit move

Preventive Maintenance 6-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 6. Press “ESC” to return the piercing unit to its original position. 7.

Preventive Maintenance BioRobot EZ1 DSP Workstation User Manual 02/2007 6-5 6. Wipe the O-rings of the tip adapters with tissue. 6.3 Weekly main

Preventive Maintenance 6-6 BioRobot EZ1 DSP Workstation User Manual 02/2007 6.4 Reagents for decontamination The following disinfectants and deter

Troubleshooting BioRobot EZ1 DSP Workstation User Manual 02/2007 7-1 7 Troubleshooting 7.1 Detecting an error If a protocol run is interrupted due

Troubleshooting 7-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 7.2 Error codes Error code Description 10 Failed to return to origins, proto

Glossary BioRobot EZ1 DSP Workstation User Manual 02/2007 8-1 8 Glossary Term Description Cartridge rack A metal rack that accommodates reagent car

Contents Contents-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 This page intentionally left blank

Glossary 8-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 Term Description O-ring A ring that is fitted at the bottom of a tip adapter. It is r

Glossary BioRobot EZ1 DSP Workstation User Manual 02/2007 8-3 Term Description Workstation door The main door at the front of the BioRobot EZ1 DSP.

Glossary 8-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 This page intentionally left blank

Appendix A BioRobot EZ1 DSP Workstation User Manual 02/2007 A-1 Appendix A Technical data Environmental conditions Power 100–120/200–240 V AC ± 10%

Appendix A A-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 Mechanical data and hardware features Dimensions Width: 0.32 m (13 in.) Height: 0.

Appendix A BioRobot EZ1 DSP Workstation User Manual 02/2007 A-3 Pipettor head Contains 6 high-precision syringe pumps, each containing a tip adapter

Appendix A A-4 BioRobot EZ1 DSP Workstation User Manual 02/2007 Labware Reagents are loaded onto the worktable using the reagent cartridges. These

Appendix A BioRobot EZ1 DSP Workstation User Manual 02/2007 A-5 QIAGEN accepts its responsibility in accordance with the specific WEEE recycling r

Appendix A A-6 BioRobot EZ1 DSP Workstation User Manual 02/2007 This page intentionally left blank

Appendix B BioRobot EZ1 DSP Workstation User Manual 02/2007 B-1 Appendix B Warranty statement Thank you for your purchase of QIAGEN instrumentation.

Safety Information BioRobot EZ1 DSP Workstation User Manual 02/2007 1-1 1 Safety Information This manual contains information and warnings that mus

Appendix B B-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 Liability clause QIAGEN shall be released from all obligations under its warranty in

Index BioRobot EZ1 DSP Workstation User Manual 02/2007 Index-1 Index C Cartridge rack, 3-8 Connector panel, 3-5 Control panel, 3-1, 3-2 E Elution tu

Index Index-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 Tip rack, 3-6 Tips, 3-6 loading, 5-11 Tray, 3-9 removing, 5-9 Troubleshooting, 7-1 W

QIAGEN Distributors and ImportersPlease see the back cover for contact information for your local QIAGEN office.ArgentinaTecnolab S.A.Tel: (011) 4555

Australia Orders 03-9840-9800 Fax 03-9840-9888 Technical 1-800-243-066Austria Orders 0800/28-10-10 Fax 0800/28-10-19 Technical 0800/28-10-11Belgium Or

Safety Information 1-2 BioRobot EZ1 DSP Workstation User Manual 02/2007 1.1 Proper use WARNING Risk of personal injury and material damage [W1]

Safety Information BioRobot EZ1 DSP Workstation User Manual 02/2007 1-3 WARNING Electrical hazard [W2] Any interruption of the protective conduc